M8265
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M8265
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Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1,2 and 3
| ISBN-10: | 0-87969-577-3 |
| ISBN-13: | 978-0-87969-577-4 |
Description
| General description | In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. It includes 240 laboratory protocols in DNA science in which over 35% were created especially for this edition, along with coverage of bioinformatics and DNA microarrays. |
Properties
| publication info | J.F. Sambrook and D.W. Russell, ed., Cold Spring Harbor Laboratory Press, 2001, 2100 pp., soft cover |
Table Of Contents
| Table of Contents | Chapter 1. Plasmids and Their Usefulness in Molecular Cloning Chapter 2. Bacteriophage lambda and Its Vectors Chapter 3. Working with Bacteriophage M13 Vectors Chapter 4. Working with High-capacity Vectors Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction Chapter 9. Preparation of Radiolabeled DNA and RNA Probes Chapter 10. Working with Synthetic Oligonucleotide Probes Chapter 11. Preparation of cDNA Libraries and Gene Identification Chapter 12. DNA Sequencing Chapter 13. Mutagenesis Chapter 14. Screening Expression Libraries Chapter 15. Expression of Cloned Genes in Escherichia coli Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells Chapter 18. Protein Interaction Technologies |
